Session WOE. There are 5 abstracts in this session.



Session: METABOLOMICS 2, time: 10:45 - 11:00 am

NMR Spectroscopic Study of [1,3- 13C2] Ethyl Acetoacetate Metabolism in Pancreatic Cancer Cells


Wirya Feizi; Lloyd Lumata
UT Dallas, Richardson, TX

Pancreatic Ductal Adenocarcinoma (PDAC) is among the most lethal of all cancer types,1,2 with approximately 48,000 new cases and 40,000 deaths annually in the United States. Tracking aberrant metabolism in cancer cells is one of the many applications of Nuclear Magnetic Resonance (NMR). Its high specificity and use of non-destructive RF waves are some reasons that we are using it in this study as a spectroscopic tool. The wide chemical shift dispersion of the 13C nucleus allows NMR spectral distinction of the specific metabolites or biochemical in cancer cells and tissues.  In this study, we have investigated the metabolism of [1,3-13C2] ethyl acetoacetate in pancreatic ductal adenocarcinoma.


Session: METABOLOMICS 2, time: 11:00 - 11:15 am

Metabolic Differences in Blood Plasma to Identify Aggressive Prostate Cancer using NMR Spectroscopy


Pradeep Kumar1; Rajeev Kumar2; Sanjay Sharma3; Sanjay Thulkar4; Siddhartha Dattagupta5; S. Senthil kumaran1; N. R. Jagannathan6; rama Jayasundar1; Virendra Kumar1
1Department of NMR and MRI Facility, AIIMS, New Delhi, India; 2Department of Urology, AllMS, New Delhi, India; 3Department of Radiodiagnosis, RPC, AIIMS, New Delhi, India; 4Department of Radiodiagnosis, IRCH, AIIMS, New Delhi, India; 5Department of Pathology, AIIMS, New Delhi, India; 6Dept. of Radiology, Chettinad Academy of Research, Kelambakkam, Tamil Nadu, India

Gleason score (GS) is currently the best clinical predictor of prostate cancer (PCa) specific mortality. This study investigates the metabolic profile of blood plasma for distinguishing PCa patients with high and low grade GS using 1H-NMR metabolomics. A significantly higher concentration of lactate, pyruvate, choline, dimethylamine, alanine, glucose, betaine; and lower concentration of leucine, isoleucine, valine, histidine phenylalanine were observed in PCa patients with GS 8 & 9 as compared to patients with GS 7 and 6. Our results suggested that the metabolic pathway alterations seen in amino acids, phospholipids and glucose may be related to changes in PCa progression. Identifying biomarker/s associated with GS can improve prediction of aggressive disease and metabolic understanding of progression of low grade cancers.


Session: METABOLOMICS 2, time: 11:15 - 11:30 am

Integration of Digital Microfluidics and NMR for In-Vivo Stress Response Monitoring in Daphnia magna


Amy Jenne1; Sebastian von der Ecken2; Vincent Moxley-Paquette1; Ian Swyer2; Ronald Soong1; Monica Bastawrous1; Falko Busse3; Wolfgang Bermel3; Daniel Schmidig4; Till Kuehn4; Rainer Kuemmerle4; Danijela Al Adwan-Stojilkovic4; Stephan Graf4; Thomas Frei4; Martine Monette5; Henry J. Stronks5; Aaron Wheeler2; Andre J Simpson1
1University of Toronto, Scarborough, Canada; 2University of Toronto, Toronto, Canada; 3Bruker BioSpin, Rheinstetten, N/A; 4Bruker Biospin AG (Switzerland), Fällanden, Switzerland; 5Bruker BioSpin Canada, Milton, ON

In-vivo NMR can provide unprecedented insight into real-time responses of organisms to contaminants. However, traditional NMR studies require a multitude of organisms and large contaminant sample reservoirs making the study of expensive contaminants or precious samples challenging. To improve in-vivo exposures studies, new methodology is introduced here that integrates digital microfluidics (DMF) with in-vivo NMR spectroscopy. Double-tuned microstrip sensors are integrated into the DMF platform to allow 1H-13C HMQC-based metabolite monitoring. The DMF-NMR system allows for Daphnia magna to be kept alive inside the spectrometer by continually supplying the sample well with new oxygenated water. This is the first demonstration of in-vivo NMR of a single Daphnid and paves the way towards a future automated environmental exposure and response platform.


Session: METABOLOMICS 2, time: 11:30 - 11:45 am

NMR chemometrics as quality control tool in ethanomedicine


Aruna Singh; Dushyant Kumar; Rama Jayasundar
Dept. of NMR, AIIMS, New Delhi, India

With increasing adverse effects of and resistance to synthetic drugs, there is growing interest in traditional plant-based medicines. However, quality control these of traditional formulations remains a challenge. In this study, proton NMR spectroscopy is used to evaluate the intra- and inter-manufacturer variabilities of traditional medicines. Three herbal tablets from three different manufacturers were studied. While NMR spectra (acquired at 700 MHz) showed intra-manufacturer uniformity, inter-manufacturer differences were also observed. NMR could be developed as a quick and easy quality control tool for complex traditional formulations.


Session: METABOLOMICS 2, time: 11:45 - 12:00

Mixture Analysis for High- and Low-polarity Organic Compounds by NMR in Sulfolane/Water and Sulfolane/]DMSO-d6 Solvents under Spin Diffusion Conditions


Francois Pedinielli; Jean-Marc Nuzillard; Pedro Lameiras
Université de Reims Champagne-Ardenne, Reims, France

The viscous sulfolane/water and sulfolane/DMSO-d6 binary NMR solvents are reported for the first time for the individualization of mixture components by spin diffusion, when molecular tumbling in solution is slow, making longitudinal cross-relaxation magnetization exchange highly efficient. All 1H nuclei resonances within the same molecule tend then to correlate in a 2D NOESY spectrum, opening the way to mixture analysis. We offer a new way to analyse mixtures by considering 31P nuclei as chemical shift markers. We report the individualization of four polar dipeptides and of four non-polar phosphorus-containing compounds respectively dissolved in sulfolane/water and sulfolane/DMSO-d6 by means of homonuclear selective 1D and 2D 1H experiments and a heteronuclear 2D 1H-31P HSQC-NOESY experiment by taking advantage from spin diffusion.