Session FOB. There are 3 abstracts in this session.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 10:40 - 10:55 am

23Na MRI and 1H MRS at 21.1T as Metrics of Stem Cell Donor Efficacy in a Stroke Model

Shannon Helsper1; Xuegang Yuan2; F. Andrew Bagdasarian1; Samuel Grant1
1NHMFL, Tallahassee, FL; 2FAMU-FSU College of Engineering, Florida State Uni, Tallahassee, Florida

Investigation of preclinical stem cell donor efficacy is imperative to translational efforts in stroke treatment. Here, we compare therapeutic impact between two human mesenchymal stem cell donors (one good, one compromised) applied to a rat model of transient ischemia. Biochemical markers were measured longitudinally over 21 day using sodium chemical shift imaging, relaxation enhanced MR spectroscopy, and T2-weighted proton imaging at 21.1 T. Although cell assays indicated normal activity at passage 5 (P5) coinciding with time of implantation, P8 assays exhibited reduced metabolic and growth levels for the compromised donor only. MR results demonstrate the ability to distinguish between donor efficacy as early as 3 days post-ischemia even when apparently healthy P5 cells were transplanted.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 10:55 - 11:20 am

Quantitative Multi-parametric MRI and in-vivo MR Spectroscopy

in the study of Breast Cancer Metabolism

Naranamangalam R Jagannathan
Chettinad Academy of Research & Education, Kelambakkam, Chennai, India

Multi-parametric quantitative MRI and MRS have played a key role in diagnosis of breast cancer and its response to therapy. Tumor volume, apparent diffusion coefficient (ADC), kinetic parameters like volume transfer coefficient estimated by routine, diffusion and dynamic contrast MRI methodologies provide information on the tumor morphology, microstructure, microenvironment and abnormal vasculature. Sequential ADC measurements as a function of various cycles of therapy is useful to predict early response compared to morphological parameters. By the use of in-vivo MRS, water-to-fat (W-F) ratio and quantitative assessment of tCho metabolite concentration, using semi-quantitative signal-to-noise ratio of choline resonance and its absolute concentration using water as an internal reference, is possible and are shown to be useful biomarkers for diagnosis and therapy assessment.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 11:20 - 11:35 am

Measuring T2* values of Metabolites In Vivo utilizing T2*-Weighted Deconvolution Localized Correlated Spectroscopy (TIDEL-COSY)

Zohaib Iqbal1; Rajakumar Nagarajan2; Andres Saucedo3; Manoj Sarma3; M. Albert Thomas3
1UT Southwestern Medical Center, Dallas, TX; 2Human Magnetic Resonance Center, IALS, UMass, Amherst, MA; 3UCLA School of Medicine, Los Angeles, CA

Magnetic Resonance Spectroscopy (MRS) excels at measuring biochemical concentrations in vivo. Earlier 1D MRS studies at 3T with short TE sequences have reported detection of a broad range of metabolites. Though, these peaks are subject to terrible spectral overlap in 1D MRS. Two-dimensional localized correlated spectroscopy (L-COSY), is a powerful technique to distinguish overlapping resonances. Recently, it has been demonstrated that spectral signals can be separated by their T2* values by using a T2* weighted deconvolution (TIDE) approach. We demonstrate that the TIDE method can be applied to in vivo L-COSY measurements, a technique termed TIDEL-COSY, to yield important T2* information which may aid in the diagnosis of various pathologies.