Session FOB. There are 4 abstracts in this session.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 10:15 - 10:40 am

Status and New Developments at BioMagResBank (BMRB)

John L. Markley1; Pedro R. Romero1; Kumaran Baskaran1; Jonathan R. Wedell1; Hongyang Yao1; Dimitri Maziuk1; Eldon L. Ulrich1; Hamid R. Eghbalnia1; Michael R. Gryk2; Chad M. Rienstra1; Jeffrey C. Hoch2
1University of Wisconsin-Madison, Madison, WI; 2UConn Health, Farmington, CT

BMRB serves the NMR community by supporting a curated archive of primary and derived data linked to scientific investigations under the “FAIR Principles” (Findability, Accessibility, Interoperability, and Reusability). BMRB is supported by the National Institutes of Health. Recent developments at BMRB include improvement of its application program interface (API), extensions of the NMR-STAR dictionary to cover recent developments in NMR experimentation, new tools for visualizing NMR data, and a new and improved data deposition system (BMRBdep) for studies of proteins and nucleic acids. Future plans are to build up the fraction of solid state NMR data in the BMRB Archive. BMRB supports studies of metabolomics and natural products through a library of 1D and 2D NMR spectra of pure compounds.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 10:40 - 10:55 am

23Na MRI and 1H MRS at 21.1T as Metrics of Stem Cell Donor Efficacy in a Stroke Model

Shannon Helsper1; Xuegang Yuan2; F. Andrew Bagdasarian1; Samuel Grant1
1NHMFL, Tallahassee, FL; 2FAMU-FSU College of Engineering, Florida State Uni, Tallahassee, Florida

Investigation of preclinical stem cell donor efficacy is imperative to translational efforts in stroke treatment. Here, we compare therapeutic impact between two human mesenchymal stem cell donors (one good, one compromised) applied to a rat model of transient ischemia. Biochemical markers were measured longitudinally over 21 day using sodium chemical shift imaging, relaxation enhanced MR spectroscopy, and T2-weighted proton imaging at 21.1 T. Although cell assays indicated normal activity at passage 5 (P5) coinciding with time of implantation, P8 assays exhibited reduced metabolic and growth levels for the compromised donor only. MR results demonstrate the ability to distinguish between donor efficacy as early as 3 days post-ischemia even when apparently healthy P5 cells were transplanted.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 11:20 - 11:35 am

Measuring T2* values of Metabolites In Vivo utilizing T2*-Weighted Deconvolution Localized Correlated Spectroscopy (TIDEL-COSY)

Zohaib Iqbal1; Rajakumar Nagarajan2; Andres Saucedo3; Manoj Sarma3; M. Albert Thomas3
1UT Southwestern Medical Center, Dallas, TX; 2Human Magnetic Resonance Center, IALS, UMass, Amherst, MA; 3UCLA School of Medicine, Los Angeles, CA

Magnetic Resonance Spectroscopy (MRS) excels at measuring biochemical concentrations in vivo. Earlier 1D MRS studies at 3T with short TE sequences have reported detection of a broad range of metabolites. Though, these peaks are subject to terrible spectral overlap in 1D MRS. Two-dimensional localized correlated spectroscopy (L-COSY), is a powerful technique to distinguish overlapping resonances. Recently, it has been demonstrated that spectral signals can be separated by their T2* values by using a T2* weighted deconvolution (TIDE) approach. We demonstrate that the TIDE method can be applied to in vivo L-COSY measurements, a technique termed TIDEL-COSY, to yield important T2* information which may aid in the diagnosis of various pathologies.

Session: QUANTITATIVE NMR / in-vivo MRS, time: 11:35 - 11:50 am

Investigation of metabolomic association with androgen sensitivity in murine prostate cancer model using dynamic metabolic imaging of hyperpolarized [1-13C]pyruvate

Aditya Jhajharia; Dexue Fu; Maninder Singh; Shu Wang; Ian Qian; Aidan Kennedy; Mohummad M. Siddiqui; Dirk Mayer
University of Maryland School of Medicine, Baltimore, Maryland, USA

This study investigates different metabolic signatures of androgen-dependent (LNCaP) and androgen-independent (CSS90) murine model prostate tumors by utilizing hyperpolarized 13C pyruvate imaging. Higher pyruvate-to-lactate conversion in CSS90 compared to LNCaP tumors were confirmed by higher glycolysis in CSS90 by measuring extracellular acidification rate (ECAR) of the tumor slices. After treatment with MDV3100, a second-generation AR antagonist, a reduced pyruvate-to-lactate conversion in LNCaP tumors was a positive response to targeted therapy and was also supported by higher oxidative phosphorylation by oxygen consumption rate measurements. These initial findings demonstrated that hyperpolarized pyruvate metabolism is a useful biomarker to characterize the tumor type-specific differences and can potentially access the responses to therapies.