Session ThOA. There are 3 abstracts in this session.

Session: in(ex)-vivo 1, time: 08:30am-09:10am

MR spectroscopy of prostate metabolism

Arend Heerschap; Thiele Kobus; Isabelle Steinseifer; Nassim Tayari; Frits van Heijster; Vincent Breukels; Tom Scheenen
Radboudumc, Nijmegen,, Netherlands
MR spectroscopy of the human prostate provides metabolic information, valuable in the diagnosis of cancer (1,2). Citrate is a major metabolite in the prostate accumulating in large amounts , but knowledge about its metabolism is limited. The signal for citrate decreases in tumor tissue while the signal for choline compounds increases. Despite the clinical potential of MRS its application is hampered by the limited robustness of the current measurement methods. We will present new approaches to overcome the limitation of these methods. In addition we will present new 13C tracking data providing a quantitative view of prostate cell metabolism supporting citrate synthesis.

Session: in(ex)-vivo 1, time: 09:10am-09:50am

Diffusion-Weighted NMR Spectroscopy In Vivo to Probe Brain Cell Structure – and Beyond

Julien Valette
CEA, Fontenay‐Aux‐Roses, France
During this presentation, we will see how basic understanding of brain intracellular metabolite diffusion as measured using diffusion-weighted NMR spectroscopy (DW-MRS) in vivo has progressed over recent years, allowing relevant interpretation of DW-MRS in terms of cellular structure. It is now increasingly clear that DW-MRS primarily reports diffusion in long and thin cellular fibers, and that some quantitative information about these fibers can be extracted by diffusion modeling. This opens new possibilities for cell-specific microstructure quantification under normal and pathological conditions, as illustrated in a mouse model of astrocyte reactivity. Furthermore, we will evoke the possibility to use DW-MRS to go beyond the sole determination of cell structure, by characterizing extracellular diffusion properties and assessing cellular compartmentation of brain lactate.

Session: in(ex)-vivo 1, time: 09:50am-10:30am

Deuterium Metabolic Imaging (DMI), a novel MR-based method for  in vivo mapping of metabolism

Robin de Graaf
Yale University, New Haven, CT
Deuterium metabolic imaging (DMI) is a novel 3D method to image metabolism of deuterium-labeled substrates in healthy or diseased human brain. DMI provides a powerful tool to reveal altered metabolism and provides completely novel information compared to standard MRI. Examples of aberrant metabolism in aggressive tumors demonstrate the unique metabolic dimension that DMI adds to the standard MRI arsenal. A range of biologically relevant, affordable deuterated substrates are available to probe multiple metabolic pathways. The robustness of DMI together with the high sensitivity, ease-of-use and affordability makes this novel technique highly relevant for 3D mapping of human brain metabolism.