Session FOA. There are 3 abstracts in this session.

Session: *Omics 2, time: 08:30am-09:10am

Integrative Phenomics for Precision Medicine and Molecular Mechanisms

Marc-Emmanuel Dumas
Imperial College of London, London, United Kingdom
The gut microbiome –the comprehensive set of bacterial genes in our guts – is now recognized as a key driver in the pathophysiology of obesity, type 2 diabetes and cardiometabolic diseases and their common low-grade inflammatory component. However, signals sent by the gut microbes to the host remain elusive. Integrating the phenome – the comprehensive set of phenotypes – with metagenomics holds promises for identification of clinically-relevant markers and elucidation of mechanisms impacted by the gut microbiome. With this strategy, we evaluated the contribution of the microbiome in hepatic steatosis by integrating 1H NMR-based metabolome and hepatic transcriptome with fecal metagenomes in non-diabetic obese women and demonstrated causality through fecal microbiota transplantations and pharmacological treatments with microbial metabolites.

Session: *Omics 2, time: 09:10am-09:50am

Real-time Live Metabolomics with 2D Heteronuclear NMR in Hierarchical Biological Systems

Sunghyouk Park
Seoul National University, Seoul, Korea
Recent studies point out the link between altered metabolism and various diseases, but metabolic monitoring of biological systems are mostly performed at fixed time points on extracted material. As NMR is a non-destructive technique, we applied heteronuclear 2D NMR to living or functional biological systems. At the cell organelle level, our results suggested previously unknown roles of p53 in maintaining mitochondrial pyruvate consumption and lactate production. Additionally, this approach identified acetyl phosphate synthesis from pyruvate in live human mitochondria. How the live metabolomic method can be expanded to hierarchical biological systems involving a protein, organelle, cell, through an entire organism, and specific examples of each system will be presented. 

Session: *Omics 2, time: 09:50am-10:30am

The Rhythms of Metabolism: A Backwards Tale in Three Acts of Translational Chronobiology Metabolomics using NMR

Arjun Sengupta1; Dania Malik1; Phil Gehrman1; Krishnaiah Saikumari1; Brian Altman2; Chi Dang3; Amita Sehgal1, 4; Aalim Weljie1
1University of Pennsylvania School of Medicine, Philadelphia, PA; 2University of Rochester, Rochester, NY; 3Wistar Institute, philadelphia, PA; 4Howard Hughes Medical Institute, philadelphia, PA
Life has evolved in the context of rhythmic daily cycles rooted in the earth’s rotation. Almost all life forms have thus adapted to circadian cycles of light/dark, temperature and nutrient availability, the study of which is chronobiology. Characterization of small molecule oscillations is challenging due to the need for quantitative, multiplexed data. NMR metabolomics is well suited for this application due to its unbiased and inherently quantitative nature. This talk will be a tale in three acts describing the interaction of metabolism with chronobiology.  Counterintuitively, the talk will work backwards by first breaking the clock (cancer), then slightly disrupting the clock in human sleep disorders (insomnia) to finally understanding normal nutrient processing by metabolite tracing in Drosophila lipids.