Session PF. There are 5 abstracts in this session.

Session: Exotica, poster number: 212
Direct Measurement of Spin Diffusion Rates
Arthur Pinon1; Basile Vuichoud2; Sami Jannin2; Lyndon Emsley1
1EPFL, Lausanne, Switzerland; 2CRMN, ISA, Villeurbanne, France
The nuclear spin diffusion rate is becoming an increasingly important parameter in many areas of solid-state NMR, in particular with the development of DNP. However, measurement of macroscopic spin diffusion rates is notoriously difficult. Here we show how to accurately measure spin diffusion rates by observing paramagnetic relaxation enhancements in samples consisting of spherical polystyrene beads with well-defined sizes with the spin diffusion rate constant being the only unknown parameter needed to fit the data. The method is versatile and can be used to determine spin diffusion rates at any temperature, for any spinning speed, and in principle for any type of sample. We also show how to measure spin diffusion rates from low temperature DNP build-up curves.

Session: Exotica, poster number: 213
Binding of ligands and small molecules to macromolecules probed by electrophoresis NMR
Ulrich Scheler
Leibniz-Institut für Polymerforschung Dresden e.V., Dresden, Germany
The binding of small ions and molecules to charged macromolecules is investigated by electrophoresis NMR. Instrumentation and strategies for data processing are discussed.
Binding is observed in the effective charge and hydrodynamic size of the various components in the aggregates, where the changes in the charge are more illustrative. For a demonstration of ion-specific effects beyond the valence various salts of polystyrenesulfonate have been studied.
To separate and quantify effects synthetic polyelectrolytes and dendrimers are studied. The same approach is applied to the binding of small peptides to synthetic polyelectrolytes and proteins.

Session: Exotica, poster number: 214

Spin-Lattice Relaxation Time in Prostate Cancer at Earth’s magnetic field


Sangwon Oh1; Jae Ho Han2; Jeong Hyun Shim1; Seong-Joo Lee1; Seong-Min Hwang1; Ingo Hilschenz1; Kiwwong Kim1
1KRISS, Daejeon, Korea, Republic of; 2Department of Pathology, Ajou University School o, Suwon, Republic of Korea
Ultra-low field T1 measurements in ex-vivo prostate tissues at Earth's magnetic field have been measured in a local hospital by a conventional induction coil based NMR apparatus. T1 was found to decrease as cancerous cell in the tissue increases. However, T1 becomes impractical as the cancer ratio is under 8 %. Additionally, we have found that T1 changes in the tissue is closely related with vessel density in the tissue. From immunohistochemical staining with an antibody against CD34, stained vessel density increases as T1 decreases. This suggest that angiogenesis process is connected to the development of the cancer.

Session: Exotica, poster number: 215
Overhauser Dynamic Nuclear Polarization at Zero Magetnetic Field
Jeong Hyun Shim; Seong-Joo Lee; Seong-min Hwang; Kwon Kyu Yu; Kiwoong Kim
KRISS, Daejeon, South Korea
We experimentally prove that Overhauser Dynamic Nuclear Polarization can be conducted at virtually zero magnetic field. Hyperfine coupling in Nitroxide radical is essential and dominant when magnetic field is nearly zero. Circularly Polarized RF, instead of linearly polarized one, induces only one of the positive and negative transitions. With two RF coils orthogonally oriented and a phase synchronized two-channel RF source, in-phase and quadrature RF field can be produced at sample space. SQUID(Superconducting Quantum Interference Device)-detected NMR system was used. In reducing magnetic field from 7 μT down to 2 nT, the NMR signal intensity enhanced with circularly polarized RF nearly remains unchanged, while that enhanced with linearly polarized RF becomes 1000 times less.

Session: Exotica, poster number: 216
Establishing SI-traceable NMR Relaxometry for use in Quantitative MRI Biomarker Validation
Karl F. Stupic; Michael Boss; Kathryn Keenan; Stephen Russek
National Institute of Standards and Technology, Boulder, CO
As quantitative MRI Biomarkers become accepted clinical practice, there is a need to ensure measurements are comparable from scanner to scanner across systems, vendors, sites, and time. The establishment of reference objects (e.g. MRI phantoms) has provided the most accepted way of minimizing variation across the multitude of differences in each MRI system. These reference objects require precise measurements of their desired MR properties with traceable uncertainty. Establishing the “traceability chain” from the fundamental SI standards (particularly time and temperature) are detailed in this presentation for an NMR system being used for traceable relaxation measurements as well as analysis and uncertainty calculations.