Session PE. There are 26 abstracts in this session.

Session: MRI/MRS, poster number: 186
High Resolution Chemical Exchange Saturation Transfer MRI at 21.1 T
Jens Rosenberg2; Tangi Roussel1, 4; Samuel C. Grant2, 3; Lucio Frydman2, 4
1NeuroSPin, Gif-sur-Yvette, France; 2National High Magnetic Field Laboratory, Tallahassee, FL; 3Department of Chemical & Biomedical Engineering, Tallahassee, FL; 4Weizmann Institute, Rehovot, N/A
The 21.1 T magnet at the National High Magnetic Field Laboratory offers many benefits in pre-clinical MRI. Here we explore the increased endogenous contrast with Chemical Exchange Saturation Transfer (CEST) using a naïve rat and a rat glioblastoma model. At this high field, CEST contrast is enhanced due to a longer water T1 and increased spectral dispersion. Using a RARE sequence with 400 Gaussian pulses and offset frequency varying between –6 and +6 ppm in 0.2 ppm increments, strong CEST contrast was seen in the tumors at 3.5 ppm likely from amines and amides. Other new exchange sites were also are visible, which verify the significant improvements of CEST MRI in healthy and diseased tissue at 21.1 T.

Session: MRI/MRS, poster number: 187
Exploring the Incorporation of Hydrophobic Gadolinium Compound into Polystyrene Nanoparticles as Potential MRI Contrast Agents
Hui Xu
Clemson University, Central, SOUTH CAROLINA
We found a simple method to incorporate Gd(TTA)3 (TTA=thenoyltrifluoroacetonate) into polystyrene (PS) nanoparticles, and we have shown that Gd(TTA)3-doped PS nanoparticles have the potential for use as both T1 and T2 MRI contrast agents. These nanoparticle contrast agents have the advantages that the concentration of Gd3+ can be easily tunable, and the PS nanoparticles have the potential to incorporate a large number of Gd3+ ions per particle.

Session: MRI/MRS, poster number: 188
Characterizing the Microscopic Heterogeneity of the Living Human Brain with Spatially Resolved 5D Diffusion-Relaxation Distributions
Joao P de Almeida Martins1; Chantal Tax3; Filip Szczepankiewicz2; Greta Eklund2; Karin Bryskhe2; Derek K Jones3; Carl-Fredrik Westin4; Daniel Topgaard1
1Lund University, Lund, Sweden; 2Random Walk Imaging, Lund, Sweden; 3Cardiff University, Cardiff, United Kingdom; 4Harvard Medical School, Boston, Massachusetts
Brain tissue is a heterogeneous anisotropic material where each millimeter-scale MRI voxel contains water in multiple environments with different cellular structures and chemical compositions. This fact complicates interpretation of conventional diffusion and relaxation MRI data in terms of microscopic tissue properties. Here we introduce a data acquisition protocol and inversion procedure to characterize the heterogeneity of the living human brain with spatially resolved 5D diffusion-relaxation distributions with the dimensions isotropic diffusivity, normalized diffusion anisotropy, diffusion tensor orientation (two dimensions), and transverse relaxation rate. Signal acquisition is performed with a diffusion-weighted EPI sequence modified for tensor-valued diffusion encoding and variable echo times, giving a 5D acquisition space with dimensions directly corresponding to the ones of the distribution.

Session: MRI/MRS, poster number: 189
Characterization of iron-based biocompatible MR contrast agents for protein interaction studies
Bhargy Sharma; Konstantin Pervushin
Nanyang Technological University, Singapore, Singapore
In the recent years, many protein-based drugs have been developed to treat conformational disorders, such as amyloidosis. However, the effects of many such drugs do not translate to humans despite extensive biophysical and chemical studies. The lag of direct correspondence in efficiency of such laboratory tested protein-protein interactions into in vivo research is a major reason for this failure. in situ study of protein interactions in animals is hindered due to lack of suitable identifiers for tracing of administered protein based drugs. In our study, we characterize different iron-based nanoparticles to be used as conjugation partners for L-PGDS protein to provide suitable MR contrast for study of its interaction with amyloids in mice.

Session: MRI/MRS, poster number: 190
Clinical Imaging Potential of FRONSAC
Nadine Dispenza; R. Todd Constable; Gigi Galiana
Yale University, New Haven, CT
Despite potential for more flexible and efficient encoding that better complements receiver geometry, the past decade of work with nonlinear gradients (NLGs) has shown relatively modest improvements on accelerated image quality. In this work we present the first experimental evidence that the previously introduced ROtary Nonlinear Spatial ACquisition (FRONSAC) can notably improve accelerated image quality, both in vitro and in humans. Furthermore, this work introduces and demonstrates a number of robust and flexible attributes which show Cartesian FRONSAC retains many desirable features of Cartesian imaging, including resilience to experimental imperfections, easily changed scan geometry, and easily controlled image contrast.

Session: MRI/MRS, poster number: 191
In vivo GlucoCEST MRI of Maternal and Fetus Glucose Uptake at 21.1 T
Shannon N. Helsper1, 2; Stefan Markovic3; Ghoncheh Amouzandeh1, 2; Tangi Roussel4; Michal Neeman3; Samuel C. Grant1, 2; Jens T. Rosenberg1, 2; Lucio Frydman1, 3
1National High Magnetic Field Laboratory, Tallahassee, Florida; 2Florida State University, Tallahassee, FL; 3Weizmann Institute, Rehovot, Israel; 4NeuroSpin Centre CEA Seclay, Gif-su-Yvette, France
The potential use of glucose Chemical Exchange Saturation Transfer (GlucoCEST) in maternal and fetal glucose uptake at 21.1-T utilizing an in vivo pregnant mouse model was investigated. Phantoms of varying pH and D-glucose concentration were used to optimize saturation offsets. The CEST profile was recorded utilizing fast spin-echo images with a train of Gaussian saturation pulses applied over a range of frequency offsets. Water Saturation Shift Reference (WASSR) was used to correct B0 drift for Z spectra and MTRasym plots. In vivo contrast was evident within the placenta at 2.4-ppm, achieving 30% contrast demonstrating ability to monitor glucose metabolism using WASSR-correction at 21.1-T with increased contrast. Modified acquisition methods and post-processing methods are underway to minimize effects of motion artifacts.

Session: MRI/MRS, poster number: 192
Low-temperature magnetic resonance imaging with 2.8 µm isotropic resolution
Hsueh-Ying Chen
NIDDK/NIH, Bethesda, MD
In this work, we demonstrate the feasibility of high-resolution 1H magnetic resonance imaging (MRI) at low temperatures by obtaining a 3D MRI image of 20 µm diameter glass beads in glycerol/water at 28 K with 2.8 µm isotropic resolution. The design of low-temperature compatible MRI apparatus including planar gradient coils and a radio-frequency microcoil will be described. Sensitivity is additionally enhanced by paramagnetic doping, optimization of the sample temperature, 3D phase-encoding of k-space data, pulsed spin-lock detection of 1H nuclear magnetic resonance signals, and reduced sampling of k-space. The actual image resolution was verified by quantitative comparisons of experimental and calculated images. Our imaging approach is compatible with dynamic nuclear polarization and preliminary results will be presented.

Session: MRI/MRS, poster number: 193
Transplantation of MPIO Labeled hMSC Aggregates in a Rodent Model
F. Andrew Bagdasarian1, 2; Shannon N. Helsper1, 2; Xeugang Yuan1; Jens T. Rosenberg2; Teng Ma1; Samuel C. Grant1, 2
1Florida State University, Tallahassee, FL; 2National High Magnetic Field Laboratory, Tallahassee, FL
Currently in several clinical trials, individual adult human mesenchymal stem cells (hMSC) are a potential treatment for ischemic stroke. To enhance the delivery and therapeutic impact, this study evaluates the potential to implant super-paramagnetically labeled hMSC aggregates directly into the ischemic brain. Based on initial injection location, aggregate penetration, dissolution and therapeutic outcome are assessed in a pre-clinical model. Notably, migration of individual hMSC from the aggregate are evident and at early time points in the ischemic brain compared to control.

Session: MRI/MRS, poster number: 194
Evaluating Helmholtz-based Electrical Conductivity Mapping at 21.1 T
Ghoncheh Amouzandeh1, 2; Frederic Mentink-Vigier1; Jens T. Rosenberg1; Samuel C. Grant1, 2
1National High Magnetic Field Laboratory, Tallahassee, FL; 2Florida State University, Tallahassee, FL
This study explores the feasibility and precision of Helmholtz-based electrical property tomography (EPT) for producing electrical conductivity maps at 21.1 T. EPT relies on the fact that tissue electrical properties alter the transmitted radio frequency field (B1). Two EPT reconstruction methods (Full-form and Phase-only) are compared using phantoms with known NaCl concentrations and measured conductivity values at 900 MHz. B1 and conductivity maps are calculated for two RF structures and modes of operation (linear birdcage and quadrature saddle). Results indicate the utility of the Full-form approach when good B1 homogeneity is available as well as the benefit of Phase-only in the presence of an inhomogeneous B1 field.

Session: MRI/MRS, poster number: 195
Assessing Lung Fibrosis Progression in Surfactant Protein C Mutants with Retrospectively-Gated UTE MRI 
Ian Stecker1, 2; Sneha Sitaraman3; Zackary Cleveland1, 2; Matthew Freeman1; Jinbang Guo1; Emily Martin4; Timothy Weaver4
1Cincinnati Children's Hospital Medical Center, Cincinnati, OH; 2Dept. of Biomedical Engr. University of Cincinnati, Cincinnati, OH; 3Molecular & Developmental Biology Graduate Program, Cincinnati, OH; 4Division of Neonatology and Pulmonary Biology, Cincinnati, OH
Pulmonary fibrosis is an often fatal disorder, for which no therapies exist to reverse or halt disease progression. To improve understanding of the mechanisms underlying disease initiation and progression it is necessary to develop 1) animal models that mimic human disease and 2) methods for quantifying regional fibrosis progression in vivo. To this end, MRI is a promising modality, but it is difficult to apply to the lungs because of low tissue density, respiratory motion, and rapid T2*. To address biological and quantitative limitations, we have developed 1) a mouse model based on a mutation that predisposes humans to lung fibrosis and 2) an ultrashort echo-time MRI protocol that overcomes T2* relaxation and eliminates respiratory motion through retrospectively-gated image reconstruction.

Session: MRI/MRS, poster number: 196
Network Analysis of Female 5xFAD Mouse Brains during Early Development of Alzheimer’s Pathology
David C. Hike1, 2; Abdol Aziz Ould Ismail3; Victor Wong2; Scott E. Boebinger1, 2; Tara N. Palin1, 2; Samuel C. Grant1, 2
1National High Magnetic Field Laboratory, Tallahassee, FL; 2Florida State University, Tallahassee, FL; 3University of Pennsylvania, Philadelphia, PA
This study utilizes DTI and graph theory as a novel way for early detection of pathology and connectivity changes related to Alzheimer’s Disease. As a function of phenotype and age, DTI studies were performed on female 5xFAD mouse brains and age-matched wild type controls at 11.75 T. Current data shows a drop in FA and a decrease in connectivity in the temporal region of the brain. Changes in the piriform and temporal areas of the brain show significant changes in various network parameters. Future work will look at expanding tractography mapping to include at least 26 regions as well as an anatomically driven network analysis.

Session: MRI/MRS, poster number: 197
Monitoring Lung Fibrosis Progression in a TGF-α-induced Mouse Model via T2* Mapping with UTE MRI
Alexander Cochran1, 2; Matthew Freeman1; Jinbang Guo1; Cynthia Davidson3; William Hardie3; Zackary Cleveland1, 2
1Center for Pulm. Img. Res., CCHMC, Cincinnati, OH; 2University of Cincinnati Biomedical Engineering, Cincinnati, OH; 3Division of Pulmonary Medicine CCHMC, Cincinnati, OH
Ultrashort echo time (UTE) MRI enables high-resolution, high-signal imaging of lung parenchyma, despite rapid T2*. Moreover, multi-echo UTE allows T2* to be mapped, providing sensitivity to the biophysical properties of lung tissue. However, to quantify T2*, respiratory motion must be mitigated. We have developed a multi-echo UTE sequence and retrospectively-gated image reconstruction that allows multi-TE imaging of free-breathing mice. Using this approach, T2* maps were obtained from a novel transgenic mouse model of lung fibrosis. The T2* of fibrotic tissue (~1.7 ms) was found to lie between lung parenchyma (0.4 ms) and non-pulmonary tissues (>3 ms). These results suggest T2* mapping with retrospectively-gated UTE will provide a useful tool to assess lung fibrosis progression in animal models and human subjects.

Session: MRI/MRS, poster number: 198
Towards Neuronal Current Imaging at Ultra-Low Field
Bragi Sveinsson; Neha Koonjoo; Bo Zhu; Thomas Witzel; Matthew Rosen
MGH - Martinos Center, Charlestown, MA
Direct detection of neuronal currents has long been a goal within MRI, with the aim of improving upon the spatial and temporal resolution of BOLD fMRI. So far, good results have been shown in phantoms but detection in vivo has proven difficult. A promising current detection technique is Stimulus-Induced Rotary Saturation (SIRS), but the BOLD signal can contaminate SIRS measurements, possibly explaining inconclusive in vivo results so far. A new sequence was developed and tested in an ultra-low-field (ULF) regime (6.5 mT) where paramagnetic effects such as BOLD are reduced and is more suited for SIRS measurements in vivo.

Session: MRI/MRS, poster number: 199
Fluorinated Silicon Quantum Dots for dual 19F MR and Fluorescence Imaging
Sha Li; Yaping Yuan; Yuqi Yang; Shizhen Chen; Xin Zhou
Wuhan Inst. of Physics and Mathematics, CAS, Wuhan, China
In recent years, a variety of 19F contrast agents (CAs) have been investigated for early diagnosis of cancer due to its rare background. However, fabricating 19F-MRI probes is a great challenge. Herein, we report a one-pot microwave synthesis of fluorinated silicon quantum dots (19FSiQDs), for accurate detection of A549 lung cancer cells and tumor. The resulted 19FSiQDs showed strong fluorescence, high 19F-MRI sensitivity, favorable biocompatibility and excellent aqueous solubility. Our experiments demonstrated that such nanoprobe significantly enhanced in vivo 19F /1H MRI contrast in rats with non-small cell lung tumor.

Session: MRI/MRS, poster number: 200
A new magnetic nanoprobe as drug carrier and T1/T2 dual mode contrast agent for MRI
Xi Huang; Shizhen Chen; Yaping Yuan; Lianhua Liu; Xin Zhou
Wuhan Inst. of Physics and Mathematics, CAS, Wuhan, China
In this abstract, multifunctional Fe3O4@SiO2@PAA-cRGD nanocomposites were designed and synthesized to load water soluble Mn-porphyrin and anticancer drug doxorubicin, which could realize both pH-responsive drug release and T1/T2 dual-mode MRI capability. In vitro cell fluorescent imagings showed that c(RGDyk)-modified nanocomposites can effectively target A549 cells. Furthermore, in vitro T1-weighted and T2-weighted MR images of A549 cells were observed. For in vivo MRI, T1 and T2 relaxation was significantly accelerated in the tumor after i.v. injection of nanocomposites. These evidences showed that the nanocomposites could be used as pH-responsive T1/T2 dual-mode contrast agent, and have the potential for the tumor-targeted MRI and drug delivery.

Session: MRI/MRS, poster number: 201
Evaluating Bound Sodium using a Novel Chemical Shift Imaging Triple Quantum Technique
Nastaren Abad1, 2; Ghoncheh Amouzandeh1, 2; Jens T. Rosenberg2; Michael G. Harrington3; Samuel C. Grant1, 2
1Florida State University, Tallahassee, FL; 2National High Magnetic Field Laboratory, Tallahassee, FL; 3Huntington Medical Research Institutes, Pasadena, CA
23Na-based MRI techniques can be used to separate sodium signal originating from the extracellular and intracellular compartments. Triple Quantum (TQ) schemes can select the coherence pathway that evolves in slow moving/restricted regimes, such as in the intracellular compartment. In this study, a novel TQ technique based on a modified chemical shift imaging sequence (CSI-TQ), which yields higher signal-to-noise ratios, is introduced for studies at 21.1 T.

Session: MRI/MRS, poster number: 202
Eddy Current Nulled Constrained Optimization of Isotropic Diffusion Encoding Gradient Waveforms
Grant Yang1; Jennifer McNab2
1Electrical Engineering, Stanford University, Stanford, CA; 2Radiology, Stanford University, Stanford, CA
A promising avenue for improving the specificity and sensitivity of diffusion MRI for characterizing the complex microenvironments of the brain is the design and optimization of gradient waveforms beyond the standard Stejskal-Tanner pulsed gradients design. While the usage of novel diffusion waveforms provides additional information about tissue microstructure, it also complicates the modeling and correction of eddy current induced image distortions. In this work, we demonstrate the design of eddy current nulled isotropic diffusion encoding pulse sequences using a constrained optimization framework. We show that correction of the principal eddy current time constants can be accomplished with a small reduction in achievable b-value.

Session: MRI/MRS, poster number: 203
Brain White Matter Fiber Configuration Analysis with Spherical Harmonic (CASH) Expansion of Diffusion Displacement Probability.
Manish Amin; Guita Banan; Luis Colon-Perez; Matthew Hey; Catherine Price; Thomas Mareci
University of Florida, Gainesville, Fl
Diffusion Weighted Imaging (DWI) has become an important tool in relating brain structure and pathology. In recent years, the Displacement Probability Function (DPF) and Fiber Orientation Distribution (FOD) function have become widely used in analyzing diffusion data. These functions can be expanded using spherical harmonic functions. Each unique fiber configuration would have a unique representative series of spherical harmonic coefficients, which can be used to determine the underlying microstructure.
To distinguish between fiber configurations using configuration analysis with spherical harmonics (CASH), the coefficients that represent a configuration must be combined in a way to make them invariant under rotation. In this way, a dictionary of known configurations can be created to compare unknown fiber configurations with.

Session: MRI/MRS, poster number: 204
Total Correlated Spectroscopic Imaging in Human Calf In-Vivo Using Continuous and Adiabatic RF pulses and Echo Planar Readout
Manoj Sarma1; Andres Saucedo1; Cathy Lee1, 2; Christine Darwin1; Ravinder Regatte3; M. Albert Thomas1
1UCLA School of Medicine, Los Angeles, CA; 2VA Greater Los Angeles Medical Center, Los Angeles, CA; 3NYU Langone Medical Center, New York, NY-10016, NY
Total correlated spectroscopy (TOCSY) is a powerful technique similar to COSY while providing additional information of relayed cross peaks. Due to the SAR issue, the potential of TOCSY and its extension to multivoxel total correlation spectroscopic imaging (TOCSI) have not been fully exploited in-vivo. Here, for the first time, we implemented a novel 4D echo planar-based TOCSI (EP-TOCSI) in human calf muscle in-vivo using three mixing strategies. Further, it was combined with acceleration technique to be evaluated in human pathologies. Results are presented from a corn oil phantom, and in-vivo data at 3T. We demonstrated that EP-TOCSI can uncover the hidden peaks of IMCL/EMCL in calf muscle which may play an important role in better estimation of degree of unsaturation.

Session: MRI/MRS, poster number: 205
Influence of Fixative Composition on the Relaxation Properties of Fixed Tissue
Prasanna Mishra; Ivan Vučković; Tarek Nayfeh; Caroline Sussman; Timothy Kline; Vicente Torres; Slobodan Macura
Mayo Foundation, Rochester, MN
The problem in fixed tissue imaging is variability of T1 and T2 relaxation times in identical tissues under similar conditions. Analyzing formalin fixatives from various sources (4% paraformaldehyde PFA) we found that the presence of salts accelerates the chemical exchange between the water and fixative hydroxyl protons. Since the salt concentration is not strictly monitored the exchange broadening of the water line seems out of control which translates into variability of the measured T2. Also, the water T2 depends on the formalin concentration. Solution to the problem is to scan fixed specimens while in PBS instead of fixative. Removal of fixative results in approximately twofold increase of T2 which may be favorable for T2 weighted and diffusion weighted methods.

Session: MRI/MRS, poster number: 206
pH Imaging in Methylmalonic Acidemia using CEST MRI of Iopamidol
Kowsalyadevi Pavuluri1; Irini Manoli3; Alexandra Pass3; Alexander Lesser3; Yuguo Li1, 2; Charles P. Venditti3; Michael T McMahon1, 2
1Johns Hopkins University School of Medicine, Baltimore, MD; 2Kennedy Krieger Research Institute, Baltimore, MD; 3National Human Genome Research Institute, Bethesda, MD
Isolated methylmalonic acidemia leads to a toxic buildup of methylmalonic acid in the blood and urine. Tubulointerstitial nephritis leading to progressive renal failure is a key disease manifestation, with no specific treatment outside dietary and supportive measures. GFR and other available serum based biomarkers are either invasive or slow in detecting kidney injury. In this study, we evaluate iopamidol, as a CEST pH sensor in a mouse model of MMA. This contrast agent can be administered to create concentration-independent pH maps based on the relative signal of two labile protons resonating at 4.2 and 5.5 ppm from water. As part of this study, we developed a CEST MRI protocol and detected changes in two metrics: filtration fraction and pH.

Session: MRI/MRS, poster number: 207
Acupuncture-MRI Cancer Probe
Rex Gerald II1; Jie Huang2; Klaus Woelk2; Ming Huang2; Lingyu Chi2; William Stoeker3; Sean Cartwright4
1American Inventor Institute, Willow Springs, IL, USA; 2Missouri University of Science & Technology, Rolla, MO; 3Dermatology Center, Rolla, MO, USA; 4Phelps County Regional Medical Center, Rolla, MO, USA
The Acupuncture-MRI Cancer Probe delineates the lateral extent and depth of skin tumors. The probe includes a radiofrequency tuned circuit with an antenna in the shape of an acupuncture needle that is positionally-controlled using a mechanical actuator to provide sub-millimeter lateral and depth resolution. The probe facilitates the complete excision of basal cell carcinomas in the first pass of the Mohs micrographic surgical procedure.

Session: MRI/MRS, poster number: 208
Brain Energetics in Acute Traumatic Brain Injury Patients: Effect of Succinate

Matthew Stovell1; Marius Mada1; Adel Helmy1; Eric Thelin1; Jiun-Lin Yan1; Mathew Guilfoyle1; Ibrahim Jalloh1; Duncan Howe1; Peter Grice1; Andrew Mason1; Susan Giorgi-Coll1; Clare Gallagher2; Michael Murphy1; David Menon1; Adrian Carpenter1; Peter Hutchinson1; Keri Carpenter1
1University of Cambridge, Cambridge, United Kingdom; 2University of Calgary, Calgary, Canada
A key therapeutic target in the critical early post-injury period of traumatic brain injury (TBI) is cell mitochondrial dysfunction, characterised by elevated brain extracellular lactate/pyruvate (L/P) ratio without hypoxia. We studied effect of microdialysis-delivered disodium succinate (12mmol/L) on brain energy state (phosphocreatine/gammaATP ratio (PCr/gammaATP)) with 31P MRS at 3T (oblique 2D CSI, 2.5x2.5x2.5cm3 voxels), and microdialysis L/P ratio in eight TBI patients. Succinate perfusion increased pyruvate (+26%, p<0.0001) and decreased L/P ratio ( 13%, p<0.0001) (lme model), but no clear-cut change in PCr/gammaATP (p>0.4). However, %decrease in L/P ratio for each patient following succinate perfusion was significantly associated with %increase in PCr/gammaATP ratio (Spearman's r = 0.86, p =0.024). Succinate may support brain energy metabolism in TBI patients with mitochondrial dysfunction.

Session: MRI/MRS, poster number: 209
Advance in in-vivo spectroscopy techniques and analysis at the Stanford Center for Cognitive and Neurobiological Imaging

Laima Baltusis
Stanford University, Stanford, CA
The Stanford Center for Cognitive and Neurobiological Imaging (CNI) develops and disseminates cognitive and neurobiological imaging methods to the human neuroimaging community. The core instrumentation is a research-dedicated 3T MRI scanner. The CNI actively develops, tests, and deploys the latest research sequences including spectroscopy sequences for GABA specific and multi-metabolite data acquisition.
CNI provides the community with data management services. CNI is transitioning to a new database management system Flywheel. A critical new feature is the ability to share computational methods within the system.
We will present examples of current studies, which illustrate data acquisition and data processing tools accessible to the user community which simplify the use and sharing of spectroscopy sequences in neuroimaging applications.

Session: MRI/MRS, poster number: 210
Ln(III)-Tagged Oligonucleotides as Paramagnetic NMR Probe to Study Nucleic Acid-Protein Interactions
Muhan He
SUNY at Albany, Albany, NY

Our research is focused on the development of paramagnetic probes that facilitate in-cell NMR experiments. These probes enhance our fundamental understanding of how nucleic acids interact with proteins inside of live cells. I developed a Dy(III)-based paramagnetic probe coordinated to a macrocyclic ligand, TCMC. The designed lanthanide probe is inert towards oligonucleotides. The probe was coupled to a stem loop oligonucleotide, SL3, a DNA analog of the packing region of HIV’s genomic RNA. Internal oligonucleotide labeling allowed us to investigate binding of HIV-1 nucleocapsid protein NCp7 using paramagnetic NMR experiments. Distance-dependent paramagnetic resonance enhancement (PRE), as well pseudo-contact chemical shift (PCS) effects have been observed during the in vitro experiments. The system is currently being extended towards in-cell NMR experiments.


Session: MRI/MRS, poster number: 211
Binomial shape-RF in magic echo sandwich sequence for imaging

Eloïse Mougel; Eric Van-Reeth; Denis Grenier
Univ Lyon, INSA‐Lyon, CNRS, Inserm, CREATIS UMR, F-69100,Lyon, France
In magnetic resonance imaging, the use of magic sandwich echo sequence (MSE) requires some precautions. It needs a radiofrequency burst (RF-BURST) which locks the spins in double rotary frame. This work investigates, experimentally and with Bloch propagation simulation, a slice selective version of MSE through different binomial RF-BURST.